TABLE 2.

Specificity of assay B^a

Bacterial species	Sourceb	Isolate origin	Resultc
Mycobacterium abscessus	NRZ	Humand	−
Mycobacterium africanum	NRZ	Humand	−
Mycobacterium avium	NRZ	Humand	−
Mycobacterium bovis	NRZ	Cattled	−
Mycobacterium chelonae	NRZ	Humand	−
Mycobacterium fortuitum	NRZ	Humane	−
Mycobacterium gordonae	NRZ	Humane	−
Mycobacterium gordonae	DITM	Humane	−
Mycobacterium kansasii	NRZ	Humand	−
Mycobacterium leprae	DITM	Humand	−
Mycobacterium malmoense	NRZ	Humane	−
Mycobacterium marinum	NRZ	Humand	+
Mycobacterium microti	NRZ	Moused	−
Mycobacterium scrofulaceum	NRZ	Humand	−
Mycobacterium smegmatis	NRZ	Humand	−
Mycobacterium szulgai	NRZ	Humand	−
Mycobacterium tuberculosis	NRZ	Humand	−
Mycobacterium ulcerans	DITM	Humand	+
Mycobacterium xenopi	NRZ	Humane	−
Escherichia coli	MVP	Humane	−
Propionibacterium acnes	MVP	Humand	−
Staphylococcus aureus	MVP	Humane	−
Staphylococcus epidermidis	MVP	Humane	−
Streptococcus pyogenes	MVP	Humand	−

^aShown are (myco)bacterial species commonly contaminating human skin and the results of the specificity testing of sequencing assay B.

^bDNA extracts that were not available at the Department of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, Munich, Germany (DITM) were provided by the National Reference Center for Mycobacteria, Borstel, Germany (NRZ), and the Max von Pettenkofer-Institut, Ludwig-Maximilians-University, Munich, Germany (MVP).

^cResults of DNA extracts subjected to assay B: +, positive PCR result; −, negative PCR result.

^dThe primary patient isolates were considered pathogenic bacteria.

^eThe primary patient isolates were considered commensals/contaminants of clinical samples.