TABLE 3.

Limits of detection of four real-time PCR assays and intra-assay precision results of 10-fold dilutions of HEV genotype 3 or 4 DNA plasmid controls tested in triplicate

DNA copy no.a	Assay A				Assay B				Assay C				Assay D
	No. of detected samples/total	Mean CT	SD	CV%b	Detected samples	Mean CT	SD	CV%b	No. of detected samples/total	Mean CT	SD	CV%b	No. of detected samples/total	Mean CT	SD	CV%b
HEV-3
100,000	3/3	24.55	0.47	1.86	3/3	25.30	0.94	3.56	3/3	30.28	0.85	2.73	3/3	26.12	0.41	1.53
10,000	3/3	28.58	0.37	1.24	3/3	28.09	0.64	2.05	3/3	33.48	0.68	1.91	3/3	29.98	0.48	1.54
1,000	3/3	31.37	0.34	1.09	3/3	32.77	0.16	0.48	3/3	37.34	0.78	2.09	3/3	32.80	0.94	2.88
100	3/3	34.91	0.23	0.65	3/3	36.35	1.32	3.53	3/3	40.78	1.17	2.90	3/3	36.74	0.59	1.64
10	3/3	37.16	0.73	1.95	2/3	39.31	1.81	4.60	0/3				1/3	39.92
HEV-4
100,000	3/3	23.78	0.66	2.77	3/3	25.86	0.18	0.69	3/3	31.85	0.17	0.53	3/3	30.77	0.90	2.82
10,000	3/3	28.44	0.39	1.36	3/3	29.25	0.72	2.31	3/3	35.86	0.12	0.32	3/3	34.03	0.33	0.93
1,000	3/3	31.59	0.68	2.16	3/3	32.81	0.09	0.24	1/3	39.54			1/3	38.21
100	3/3	34.53	0.56	1.64	3/3	36.24	0.58	1.53	0/3				0/3
10	3/3	37.36	0.13	0.35	2/3	40.22	0.51	1.26	0/3				0/3

^aNumber of plasmid DNA copies per reaction.

^bCV%, coefficient of variation.