TABLE 5.
Detection of HEV RNA by conventional nested RT-PCR and real-time RT-PCR assays in 20 pig field samples
| Sample | Detectiona |
||||
|---|---|---|---|---|---|
| Real-time RT-PCR assay |
Conventional nested RT-PCR | ||||
| A | B | C | D | ||
| 19482 | − | 35.69 | − | − | + (w) |
| 19702-C | − | 36.18 | − | − | + (w) |
| 19714 | − | 36.00 | − | − | + (w) |
| 19762-B | 33.02 | 34.46 | − | − | +b |
| 19775 | 36.97 | − | − | − | − |
| 19913 | 33.04 | 35.51 | 33.62 | − | +b |
| 19903-A | − | 35.63 | − | − | − |
| 19912-B | 36.06 | − | − | − | + (w) |
| 19955 | 37.41 | 36.06 | − | − | − |
| 20354-A | 35.99 | 38.99 | − | − | − |
| 20361-A | 36.40 | − | − | − | + |
| 20383-B | 36.39 | 33.32 | − | − | − |
| 20513 | 36.13 | − | − | − | − |
| 20517 | 36.88 | − | − | − | + |
| 20613 | − | 36.78 | − | − | + (w) |
| 20467-E | 36.59 | 38.05 | − | − | + |
| 20468-D | 36.31 | 37.84 | − | − | +b |
| 20777-B | 36.52 | 35.86 | − | − | − |
| 20792-B | 37.23 | 36.46 | − | − | + (w) |
| 20855 | 35.93 | − | − | − | − |
a
−, HEV RNA-negative sample; +, HEV RNA-positive sample; w, weak reaction.
b
HEV genotype 3 by sequencing.