TABLE 2.
NS5b subtyping results for clinical samples and the mutations in the corresponding NS3 protease revealed by DNA sequencing
| Sample no. | Subtype | Amino acid mutations in NS3 proteasea |
|---|---|---|
| S2546 | 1b | S7A, R26K, V48I, Q86L, S122G, V170I |
| S2925 | 1b | I18V, R26K, V48I, L94 M, V132I, S147L, A150V |
| S2964 | 1b | R26K, E32G, L94 M, D103A, S122G, V132I, S147L, A150V |
| S2979 | 1b | S7A, L14V, C16T, I18V, R26K, Q28E, V48I, C52 M, I71V, T72C, Q80K, Q86P, A87S, H110E, V116A, G124A, S125A, V132I, Y134T, L143I, L144 M, A150V, I153L, A166S, V167L, V170I, S174N |
| S2981 | 1b | S7A, L14V, C16T, I18V, R26K, Q28E, C47S, V48I, C52V, I71V, T72C, T72I, Q80K, Q86P, A87S, V107I, H110E, V116A, S122N, G124A, S125A, V132I, Y134T, L143I, L144 M, A150V, L153L, A166S, V167L, V170I, S147N |
| S3063 | 1b | R26K, V48I, S61A, L94 M, T98A, S122G, V132I, S147L, A150V, V170I |
| S3064 | 1b | I17T, R26K, L94 M, S122G, V132I, S147L, A150V |
| S11242 | 1b | V132I, S147L, A150V |
| S11329 | 1b | R26K, Q86P, T95E, S122G |
| S11367 | 1b | R26K, V48I, A59F, L64F, Q80R, L94 M, S122G, V132I, S147L, A150V |
| S11368 | 1b | S7A, R26K, Q41H, V48I, T108A |
| S11379 | 1b | R26K, S122G, V132I, S147L, A150V |
| S11451 | 1b | T22P, R26K, A59F, L64F, Q80R, L94 M, S122G, S147L, A150V |
| S11478 | 1b | R26K, V48I, I71V, Q86P, S122G |
| S11479 | 1b | R26K, V132I, S147L, A150V |
| S11542 | 1b | R26K, V48I, I71V, Q86P, T95A, S122G |
| S11543 | 1b | R26K |
| S11562 | 1b | R26K, S61A, Q80K, L94 M, L104P, D121G, S122G, V132I, S147L, A150V |
| S11564 | 1b | R26K, S122G, V132I, A150V |
| S11614 | 1b | R26K, L94 M, S122G, V132I, S147L, A150V |
| S11615 | 1b | S7A, R26K, V48I, S61P, Q86P, P89S, V132I |
| S11675 | 1b | R26K, S61T, Y105F, S122G, V132I, S147L, A150V |
| S11676 | 1b | R26K, L94 M, S122G, V132I, S147L, A150V |
| S11708 | 1b | R26K, L94 M, S122G, V132I, S147L, A150V |
| S11710 | 1b | R26K, L94 M, V132I, S147L, A150V |
| S11718 | 1b | R26K, V48I, L94 M, S122G, V132I, S147L, A150V |
| S11750 | 1b | R26K, S122G, V132I |
| S11775 | 1b | R26K, L94 M, S122G, V132I, S147 M, A150V |
| S11823 | 1b | R26K, L94 M, S122G, V132I, S147L, A150V |
| S2662 | 2a | R26K, Q29P, V33I, S40T, T47S, G66S, L67S, K98T, I132L, V150A, L153I, T174A |
| S2828 | 2a | R26K, V33I, I35V, S40T, T47S, S49A, L67S, K98T, I132L, P146S, V150A, L153I, T174A |
| S3016 | 2a | R26K, V33I, I35V, S40T, T47S, S49A, V51I, L67S, S87G, K92R, K98T, I132L, V150A, L153I, T174A |
| S11244 | 2a | A16T, R26K, V33I, I35V, S40T, T47S, Y56F, L67S, K98T, I132L, V150A, L153I |
| S11369 | 2a | R26K, V33I, I35V, S40T, T47S, S49A, Y56F, L67S, K98T, I132L, V150A, L153I, T174A |
| S11477 | 2a | R26K, V33I, I35V, S40T, T47S, L67S, S77G, K98T, V107I, N110D, A126V, I132L, P146S, V150A, L153I |
| S11480 | 2a | R26K, V33T, I35V, S40T, T47S, L67S, K98T, I132L, V150A, L153I, T174A |
| S11528 | 2a | T22S, R26K, I35V, S40T, T47S, S49A, L67S, K98T, I132L, V150A, L153I, T174A |
| S11782 | 2a | R26K, V33I, Q34R, S40T, T47S, S49A, L67S, K98T, I132L, V150A, L153I, T174A |
a
The sequence of Con1 NS3 protease is used as the reference sequence for comparison with the sequences derived from subtype 1b clinical samples, while the sequence of strain JFH1 NS3 protease is used as the reference sequence for comparison with the sequences derived from subtype 2a clinical samples.