FIG 2.

Impact of cell culture-adaptive mutations and reversions on viral replication in Huh-7.5 cells. (A) Infectious virus released by cells 2 days after transfection with the indicated HCV RNA. Values shown represent the means ± SD of 2 (H77S) or 4 (all others) FFU assays from two independent experiments. An asterisk indicates P < 0.03 for the difference in yield from H77S.2 by two-sided Mann-Whitney test. (B) qRT-PCR analysis of the fold increase in H77S versus H77S.2 RNA relative to H77S/AAG RNA (lethal NS5B mutant) following transfection of Huh-7.5 cells. (C) Fold increase of H77S.2 mutant RNAs, as described for panel B. (D) Immunofluorescence detection of HCV core protein in cells transfected with H77S.2 versus H77S.2/12204T RNA.