FIG 6.

The N-terminal binding residues of EBNA3C are sufficient for suppressing H2AX transcription. HEK293 cells were transfected with 10 μg of pGL3-H2AX and 1, 2, and 4 μg of EBNA3C truncations (EBNA3C aa 1 to 992, aa 1 to 365, aa 366 to 620, and aa 621 to 992). The N-terminally deleted region of EBNA3C (ΔEBNA3C-N) was also included. Data from the reporter assays are represented as relative luciferase units (RLU), with means and standard deviations represented by error bars. The fold activation was calculated by comparison of the promoter activity in the presence of the pGL3-H2AX promoter with the value of the pA3M vector alone.