FIG 4.

Immunization with Ad26 and Ad35 vectors did not increase foreskin inflammation 1 year after vaccination. Foreskin specimens obtained 1 year after SIV/SHIV infection or Ad35/Ad26-based SIV immunization were evaluated immunohistochemically for CD3, CD4, HLA-DR, and Langerin expression using the objective scoring criteria (Table 1) as detailed in Materials and Methods. Mean objective scores and representative immunohistochemistry slides are presented for infected animals (A), vaccinated animals (B), and sham-vaccinated animals (C). (D) The magnitudes of IFN-γ positive Ad26 vector-specific CD4⁺ T cells were quantified in paired peripheral blood and foreskin lymphocytes via ICS after stimulation with a pool of Ad26 hexon peptides (hexon) or empty Ad26 vectors (Ad26). IEL, intraepithelial lymphocytes; LP, lamina propria. Error bars represent the SEM.