FIG 5.

Infection and vaccination elicit SIV Gag-specific CD4⁺ and CD8⁺ T cells in foreskin. Paired PBMC and foreskin lymphocytes obtained 1 year after SIV/SHIV infection or Ad35/Ad26-based SIV immunization were assessed for SIV-specific CD4⁺ and CD8⁺ T cell responses via ICS using pooled SIVmac239 Gag peptides. Flow panels in panel A depict presence of SIV Gag-specific CD8⁺ T cells in PBMC and foreskin of one representative SIV-infected animal. The magnitude of SIV Gag-specific CD4⁺ and CD8⁺ T cell responses in all SIV/SHIV-infected animals is quantified for both CD4⁺ T cells and CD8⁺ T cells in panels B and C, respectively. Similarly, flow panels in panel D depict presence of SIV Gag-specific CD8⁺ T cells in the PBMC and foreskin of a representative vaccinated animal. The magnitude of SIV Gag-specific CD4⁺ and CD8⁺ T cell responses in all vaccinated animals is quantified for both CD4⁺ T cells and CD8⁺ T cells in panels E and F, respectively. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-tailed Student t test) for comparison of the magnitude of SIV Gag-specific responses between PBMC and foreskin in Total (Tot), central/transitional memory (CM/TM), or effector memory (EM) CD4⁺ or CD8⁺ T cells. Error bars represent the SEM.