TABLE 3.
Efficacy of immunizations evaluated at 7 days after the sixth immunization obtained with protocols B, D, I, and L
| Ag | Protocol | Mean titera |
Downregulation |
||
|---|---|---|---|---|---|
| IgA | IgG | Ex vivob | In vitroc | ||
| FHV-ECL1 | B | 20 | 100 | 39 | ND |
| D | 60 | 300 | 32.5 | ND | |
| I | 60 | 900 | 53 | 69 | |
| L | 250 | 1,300 | 69.6 | 75 | |
| FHV-ECL2 | B | 20 | 100 | 33.3 | ND |
| D | 66 | 300 | 49 | ND | |
| I | 10 | 433 | 67 | 53 | |
| L | 300 | 1,235 | 67 | 62 | |
| FHV-Nter1 | B | 0 | 30 | 22 | ND |
| D | 0 | 200 | 25.7 | ND | |
| I | 30 | 1,500 | 39 | 37 | |
| L | 100 | 750 | 43 | 43 | |
| FHV-Nter2 | B | 0 | 0 | 0 | ND |
| D | 0 | 200 | 0 | ND | |
| I | 0 | 50 | 0 | ND | |
| L | 0 | 150 | 0 | ND | |
| FHV (CTRL) | B | 0 | 0 | 0 | ND |
| D | 0 | 0 | 0 | ND | |
| I | 0 | 0 | 0 | ND | |
| L | 0 | 0 | 0 | ND | |
a
The values are mean titers of binding antibodies to the different CCR5 domains, expressed as a serum dilution of 1/n (where n is the number shown).
b
Ex vivo CCR5 downregulation is expressed as percent reduction of CCR5 molecules on the surfaces of murine PBMC from immunized animals.
c
In vitro CCR5 downregulation was induced in the U937 cell line by murine antisera, and it is expressed as percent receptor internalization obtained at a serum dilution of 1/40. ND, not detectable.