FIG 10.

Inhibition of NLRX-1 or mitochondrial ROS blocks RV-induced NOX-1 expression. (A) 16HBE14o− cells were transfected with NT or NLRX-1 siRNA, infected with sham control or RV, and incubated for 16 h. (B and C) 16HBE14o− cells or mucociliary differentiated cells were infected with RV or sham control in the presence or absence of Mito-Tempo and incubated for 16 h. Total RNA was harvested, and the expression of NOX-1 mRNA was determined by qPCR and expressed as the fold change over the GAPDH housekeeping gene. The data represent means and SD calculated from 3 independent experiments (*, P ≤ 0.05; ANOVA).