FIG 8.

RV promotes NLRX-1 translocation to mitochondria in primary airway epithelial cells. (A and B) Mucociliary differentiated primary airway epithelial cells were fixed and immunolabeled with a mixture of antibodies to occludin (green) and NLRX-1 (red). Panel B is a Z section of panel A showing NLRX-1 on the apical surface and in the cytoplasm. The arrows indicate colocalization of NLRX-1 with occludin. (C and D) Mucociliary differentiated primary airway epithelial cells were apically infected with sham control or RV, incubated for 24 h, fixed, immunolabeled with antibodies to NLRX-1 (red) and MT-CO1 (green), counterstained with DAPI, and subjected to confocal microscopy. The images represent optical sections taken at a depth of 9 μm to show both NLRX-1 and MT-CO1, presumably in the middle of the cells. The total thickness of cell cultures was approximately 20 μm. The inset in panel D is a magnified view of the boxed area. All images are representative of three independent experiments.