FIG 9.
RV-stimulated mitochondrial ROS is required for RT reduction in primary airway epithelial cells. Mucociliary differentiated primary airway epithelial cells were apically infected with sham control or RV in the presence or absence of 50 μM Mito-Tempo and incubated for 24 h. (A) Cells were incubated with MitoSox Red and analyzed by flow cytometry to determine the levels of mitochondrial ROS. (B) RT was measured and expressed as a percentage of sham-infected vehicle-treated controls. The data represent means and SD calculated from three independent experiments (*, P ≤ 0.05; ANOVA).