FIG 7.

Specific knockdown of SEPT6 in DFCs/KV cells causes specific defects in LR patterning. (A) Representative image of an embryo after injection of fluorescence-labeled membrane dye at the 256-cell stage. This labeling experiment demonstrated that the injection was targeted to the intended location. (B and C) DFC architecture was not affected during the gastrula stage in the embryos injected with sept6-tMO at the 256-cell stage compared with the control embryos. (D and E) sept6-tMO injection at the 256-cell stage caused inverted patterning of visceral organs (E), in contrast to std-MO injection (D). (F to H) Similar to the control embryos (F), embryos injected with sept6-tMO at the 256-cell stage (H) showed fewer global defects than those injected at the 1-cell stage (G).