FIG 9.

sept6 is required for ciliogenesis and the Shh signaling pathway in the zebrafish neural tube. (A to C) Immunofluorescence analysis of acetylated α-tubulin on longitudinal cryosections of embryos at 2 dpf from control embryos (A), sept6-tMO morphants (B), and sMO morphants (C). The cilium area in the neural tubes is boxed. (D to L) WISH and real-time PCR analysis of Shh signaling components. Expression of shh was comparable in the control embryos and the morphants at 24 hpf (D, E, and F). In contrast, the expression levels of gli1, nkx2.2, and ptch1 in the central nervous system of sept6-tMO morphants (D, H, J, and L) were reduced in comparison to the control embryos (D, G, I, and K). **, P ≤ 0.01; N.S., not significant. Arrows (G to L) indicate the central nervous system.