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Primers used in this study
| Assay and primer target gene | Primer directiona | Primer sequence (5′–3′) |
|---|---|---|
| RT-PCR: sept6 | F | CGGCCACTGAGATAGCACGACAA |
| R | CATCTGATTTGGCAATGATGGG | |
| Real-time PCR | ||
| shh | F | TACGAGGGCAAGATAACGC |
| R | ACAGAGATGGCCAGCGAG | |
| nkx2.2 | F | CAGCATCCAATACTCATTACAC |
| R | CTTCTTACCAGAGTCGCTG | |
| gli1 | F | CTACCAGCTCTCTCAGCAAC |
| R | GCAGGACATTCCAGTGACTC | |
| ptch1 | F | CAGAGTTTGACTTCATCATGAG |
| R | CGTTGTTAGCAGGTACAACC | |
| β-actin | F | CGGAATATCATCTGCTTGTAA |
| R | CATCATCTCCAGCGAATC |
F, forward; R, reverse.
