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. 2014 Apr;34(7):1349–1362. doi: 10.1128/MCB.01052-13

FIG 4.

FIG 4

Accumulation of TIN2 at telomeres is reduced in TRF1-deficient MEFs. (A) An anti-TRF1 antibody was used to detect TRF1 protein in immunoblots in TRF1F/F MEFs with or without Cre treatment (72 h). (B) Endogenous TIN2 (left) and endogenous Rap1 (right) were detected by IF in combination with telomeric FISH (green) with or without Cre treatment (72 h). Triton X-100 was used to extract soluble proteins. DNA is stained with DAPI (blue). Bars, 1.5 μm. (C) An anti-TRF2 antibody was used to detect TRF2 protein in immunoblots in TRF2F/F MEFs with or without Cre treatment (72 h). *, unspecific bands. (D) Endogenous TIN2 (left) and endogenous TRF1 (right) were detected by IF in combination with telomeric FISH (green) with or without Cre treatment (72 h). Triton X-100 was used to extract soluble proteins. DNA is stained with DAPI (blue). Bars, 1.5 μm.