FIG 9.
Fusion of the Rap1 RCT domain to TIN2-L247E restores telomere association. (A) Indirect immunofluorescence (IF) for the indicated FLAG-HA2 TIN2 alleles (anti-HA antibody, red) and telomeric FISH (green) in TIN2−/− MEFs (72 h post-Cre). Soluble proteins were extracted by Triton X-100 treatment prior to fixation. DNA is stained with DAPI (blue). Bars, 1.5 μm. (B) Telomeric DNA ChIP for the FLAG-HA2 TIN2 alleles (anti-HA), TRF1, and Rap1 in TIN2F/F MEFs with or without Cre treatment (72 h). (C) Quantification of the ChIP signals from three identical experiments, as performed for panel B. ChIP signals were normalized to the input, and the background (PI) was subtracted. **, P < 0.05 (paired Student's t test).