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. 2014 Apr;34(8):1438–1451. doi: 10.1128/MCB.01584-13

FIG 5.

FIG 5

Rps28b binds directly to Edc3 but not to Dcp1. (A) Analysis of direct two-hybrid interactions between Rps28b and Dcp1, Dcp2, Edc3, or itself in the wild-type tester strain GGY1::171 or isogenic tester strains harboring edc3Δ (SYY1773) or dcp2-N245 (SYY2390) alleles. (B) Analysis of two-hybrid interactions between Rps28b and different fragments of Edc3 in the wild-type tester strain or the tester strain harboring an edc3Δ allele. (Top) Schematic representation of the Edc3 domain structure and EDC3 fragments used in the experiment. In both panels A and B, a DNA fragment encoding Rps28b was fused to GAL4(DB). This GAL4-DB-RPS28B fusion was used in the yeast two-hybrid system to test for Rps28b interaction with full-length Dcp1, Dcp2, Edc3, and Rps28 (A) or fragments of Edc3 (B) fused to GAL4(AD). In each case, a GAL4(DB) fusion and a GAL4(AD) fusion were cotransformed into the indicated tester strains. Individual transformants were selected, and qualitative β-galactosidase activity was determined on X-Gal-containing plates. Blue colony color indicates an interaction, and white colony color indicates no interaction.