TABLE 1.
B cell deficiency and impairment of B cell maturation are comparable in SPPL2a−/− and SPPL2a/b double-deficient micea
| Cell type | Phenotype | Mean ± SD |
P (SPPL2a−/− [Do-ko]) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type |
SPPL2a−/− |
SPPL2b−/− |
SPPL2a−/− SPPL2b−/− |
|||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | |||
| Bone marrow (% viable cells) | ||||||||||
| B cells | B220+ | 17.7 | 5.1 | 19.6 | 5.4 | 19.4 | 5.5 | 16.0 | 7.1 | >0.05 |
| Pro-/pre-B cells | B220+ IgM− | 9.1 | 3.5 | 14.1 | 4.5 | 10.8 | 3.3 | 11.4 | 4.4 | >0.05 |
| Immature B cells | B220+ IgM+ | 3.0 | 0.9 | 4.8 | 1.5 | 3.2 | 1.1 | 4.2 | 2.8 | >0.05 |
| Recirculating B cells | B220high | 5.6 | 2.2 | 0.6 | 0.3 | 5.3 | 2.3 | 0.4 | 0.2 | >0.05 |
| Spleen (% viable cells) | ||||||||||
| B cells | B220+ | 54.4 | 1.9 | 23.5 | 2.8 | 53.3 | 4.3 | 22.3 | 4.6 | >0.05 |
| T1 transitional | B220+ CD21low CD24high | 8.8 | 2.8 | 14.5 | 1.4 | 8.4 | 2.0 | 14.2 | 3.3 | >0.05 |
| T2 transitional | B220+ CD21high CD24high | 9.3 | 2.5 | 0.4 | 0.3 | 10.9 | 2.5 | 0.2 | 0.2 | >0.05 |
| Mature B cells | B220+ CD21low CD24low | 32.1 | 2.6 | 3.3 | 2.3 | 30.0 | 2.7 | 2.6 | 1.0 | >0.05 |
| Spleen (106 splenocytes) | ||||||||||
| B cells | B220+ | 55.7 | 7.7 | 8.5 | 4.5 | 47.3 | 19.6 | 5.3 | 1.4 | >0.05 |
| T1 transitional | B220+ CD21low CD24high | 9.1 | 3.0 | 5.2 | 2.8 | 7.6 | 3.9 | 3.4 | 1.0 | >0.05 |
| T2 transitional | B220+ CD21high CD24high | 9.5 | 2.2 | 0.2 | 0.1 | 9.5 | 4.1 | 0.05 | 0.03 | >0.05 |
| Mature B cells | B220+ CD21low CD24low | 32.9 | 5.4 | 1.3 | 1.2 | 2.7 | 1.1 | 0.6 | 0.3 | >0.05 |
| Lymph nodes (% viable cells) | ||||||||||
| B cells | B220+ | 20.5 | 5.3 | 6.1 | 5.1 | 23.9 | 6.5 | 3.0 | 1.0 | >0.05 |
| Lymph nodes (106 cells) | ||||||||||
| B cells | B220+ | 9.1 | 5.7 | 0.5 | 0.4 | 5.1 | 2.0 | 0.2 | 0.2 | >0.05 |
| Peritoneal cells (% viable cells) | ||||||||||
| B cells | B220+ | 37.6 | 12.9 | 7.5 | 4.2 | 38.2 | 9.1 | 5.5 | 1.8 | >0.05 |
| B1 | B220neg/low CD19high | 22.3 | 8.3 | 7.1 | 4.2 | 24.1 | 2.8 | 5.1 | 1.5 | >0.05 |
| B2 | B220high CD19+/low | 15.3 | 10.3 | 0.4 | 0.3 | 14.0 | 8.6 | 0.4 | 0.4 | >0.05 |
a
B cell subsets were analyzed by flow cytometry in bone marrow, spleen, lymph node, and peritoneal lavage samples of wild-type, SPPL2a−/−, SPPL2b−/−, and SPPL2a/b double-deficient (SPPL2a−/− SPPL2b−/−/do-ko) mice. The proportions of cell populations are indicated as the percentages of viable cells (PI−) or absolute cell numbers, respectively. Results represent means from 6 mice per genotype. Data were analyzed by one-way ANOVA with Bonferroni post hoc testing.