FIG 4.
Gene expression in the PVH and the heart. mRNA levels were measured by qPCR in WT, NCoRΔID/ΔID (ΔID), Src-1−/−, and NCoRΔID/ΔID Src-1−/− (ΔID/Src-1−/−) mice following 21 days of a LoI/PTU diet (PTU) and a LoI/PTU diet with T3 replacement for 21 days with increasing doses of T3 (PTU + T3) compared with the control (Chow). (A) Trh mRNA was measured in the PVH microdissected from mouse brain and was normalized to expression of 18S rRNA. (B) mRNA expression of known positive and negative TH targets in the heart. HCN2, Serca2, Myh6, and Myh7 were normalized to expression of cyclophilin. (A and B) n = 5 to 7. The data are presented as means and SEM and were analyzed by 2-way ANOVA with a Bonferroni post hoc test. ***, P < 0.001 versus chow and PTU + T3.