FIG 7.

Requirement for Taf9 CRD for PIC assembly. Recruitment of Spt15-Myc₁₃ (TBP), Sua7-Myc₁₃ (TFIIB), and Rpb3-Myc₁₃ (RNAP II) was determined at the core promoters of Gcn4-dependent BNA1, TRP3, and MET28 genes under SM- and non-SM-treated conditions, as well as at the Gcn4-independent RPS5 and GPM1 core promoters under non-SM-treated conditions, by ChIP assays using anti-Myc antibody. For each analysis, enrichment values were obtained with reference to a nonspecific POL1 coding sequence probe. Data from three chromatin immunoprecipitation assays from at least two independent chromatin preparations are plotted. The error bars indicate SEM.