Minimum spanning tree (MST) analysis of molecular features of 113 M. pneumoniae samples from Beijing, China, and Sydney, Australia. Each circle represents a particular MLVA profile, designated by MLVA pattern and Dégrange type in parentheses (*, novel MLVA type). The color of the circles corresponds to sample source: white for Beijing, China, and gray for Sydney, Australia. R indicates the presence of macrolide resistance, and the number in parentheses indicates the number of the resistant samples out of the total in a particular MLVA type. The size of the circle is proportional to the number of samples belonging to the indicated MLVA type. The distance between neighboring MLVA types is expressed as the number of allelic changes and is indicated by 1, 2, and 3 for one, two, and three changes, respectively.