TABLE 1.
Bacterial strains used to test the specificity of the real-time PCR (qPCR) assay for the detection of M. hyorhinis
| Organism | Strain(s)a | No. tested (n = 63) | qPCR result |
|---|---|---|---|
| Mycoplasma hyorhinis | RS ATCC 25021 and 28 field strains | 29 | + |
| Mycoplasma hyopneumoniae | RS ATCC 25934 and 3 field strains | 4 | − |
| Mycoplasma hyosynoviae | RS ATCC 25591 and 4 field strains | 5 | − |
| Mycoplasma flocculare | RS ATCC 27399 and 2 field strains | 3 | − |
| Mycoplasma hyopharyngis | RS ATCC 51909 | 1 | − |
| Mycoplasma gallisepticum | Field strain | 1 | − |
| Mycoplasma synoviae | Field strain | 1 | − |
| Mycoplasma orale | Field strain | 1 | − |
| Mycoplasma glycophilum | Field strain | 1 | − |
| Mycoplasma genitalium | Field strain | 1 | − |
| Mycoplasma bovis | Field strain | 1 | − |
| Pasteurella multocida subsp. multocida | Field strains | 2 | − |
| Haemophilus parasuis | Field strains | 4 | − |
| Actinobacillus pleuropneumoniae s9 and 11 | RS CVJ 13261, RS 56153 | 2 | − |
| Actinobacillus rossii | RS CCUG 44312 | 1 | − |
| Actinobacillus lignieresii | RS ATCC 49236 | 1 | − |
| Bordetella bronchiseptica | Field strain | 1 | − |
| Escherichia coli | RS ATCC 25922 | 1 | − |
| Staphylococcus aureus | Field strain | 1 | − |
| Streptococcus porcinus | RS ATCC 43138 | 1 | − |
| Streptococcus suis | RS ATCC 43765 | 1 | − |
a
RS, reference strain; ATCC, American Type Culture Collection, Rockville, USA; CCUG, Culture Collection, University of Göteborg, Sweden.