TABLE 3.
Primers used for the M. hyorhinis real-time PCR assay, for the sequencing of the p37 gene, and for the MLST scheme
| Gene | Primer | Sequence (5′→3′)a | Start position (in HUB-1 genome) | Amplicon size (bp) | Seq. sizeb (bp) |
|---|---|---|---|---|---|
| p37 gene | Mhr-p37-RT-F | TATCTCATTGACCTTGACTAAC | 768070 | 89 | |
| Mhr-p37-RT-R | ATTTTCGCCAATAGCATTTG | ||||
| Mhr-p37-Probe | FAMa-CATCCTCTTGCTTGACTACTCCTG-BHQ1b | ||||
| p37-A1F | TTTTCCTTGGCCTGATGAAC | 766864 | 836 | 1,278 | |
| p37-A1R | TTCAGATCCACTTGTAAAAATTGC | ||||
| p37-A2F | AGCTTGAACAGTTGGATCGT | 764 | |||
| p37-A2R | GGGTGCTGTAAAAGGCTGAG | ||||
| dnaA | dnaA_F | CCAGAAGTCTTAGGTGGTTTTGA | 612 | 495 | 435 |
| dnaA_R | ATGATCCTTGCCTCCAAAAA | ||||
| rpoB | rpoB_F | CAACGTCAAGCTGTTCCATT | 71189 | 563 | 496 |
| rpoB_R | CCTGCACTAACTTCTGATCCAA | ||||
| gyrB | gyrB_F | CCGATTCTGATGGTTCACAT | 316687 | 399 | 291 |
| gyrB_R | TTTTTGCATATTTTGCATTTTCTT | ||||
| gltX | gltX_F | GCTGAAAGACTCTCAAAATCACC | 415737 | 493 | 370 |
| gltX_R | CAAGCCTTTTTGAAATTAGTTCTTT | ||||
| adk | adk_F | GCGATGGCATCTAATTCTTTT | 488754 | 484 | 277 |
| adk_R | ACTCAGGCAAAGTTTTTAGAACA | ||||
| gmk | gmk_F | TTGCGCCTGTTTCTGTTAAT | 522045 | 499 | 435 |
| gmk_R | AAGAGACAAAAGACCTAATGAAGTAGA |
a
FAM, 6-carboxyfluorescein, fluorescence reporter dye; BHQ, Black Hole Quencher.
b
Size of sequenced fragment.