TABLE 2.
Performance characteristics of molecular mycobacterial detection compared with those of culture
| Assay name and type of detection | Sensitivity (%) (95% CI) | Specificity (%) (95% CI) | PPV (%) (95% CI) | NPV (%) (95% CI) |
|---|---|---|---|---|
| MTBDRplus (v1.0), prospective MTBC | 0.79 (0.60–0.92) | 0.99 (0.92–1.00) | 0.96 (0.79–0.99) | 0.92 (0.84–0.97) |
| Anyplex | ||||
| Retrospective MTBC | 0.86 (0.68–0.96) | 0.99 (0.92–1.00) | 0.96 (0.80–0.99) | 0.95 (0.87–0.98) |
| Retrospective NTM | 1.00 (0.69–1.00) | 0.97 (0.91–0.99) | 0.77 (0.46–0.95) | 1.00 (0.96–1.00) |
| Prospective MTBC | 1.00 | 0.96a (0.89–1.00) | 0.93a (0.83–1.00) | 1.00 |
| Prospective NTM | 1.00 | 0.97b (0.92–1.00) | 0.75b (0.44–1.00) | 1.00 |
a
All 3 MTBC culture-negative samples were from patients with a history of tuberculosis. One sample (smear positive) was from a patient for whom samples sent 2 months earlier and 3 months later were M. tuberculosis culture positive, one sample (smear negative) was from a patient for whom four specimens had been submitted in 1 week, with only one of the four samples being culture positive (not the one tested by PCR), and one sample (smear negative) was from a patient for whom a sample sent 3 weeks previously was M. tuberculosis and PCR positive.
b
All were microscopy positive.