TABLE 1.

Geographical origin and molecular results concerning the PCR and high-throughput sequencing technologies of the 22 MCPyV strains studied

Participant no.	Sample	Geographic origin	Age (yr)	Sexa	PCR results for fragment:			Phylogeny (1,284-bp fragmentb)
					A	B	C
1	AmePIc	South America (Amerindians)	35	F	+	+	+	+
2	AmePA	South America (Amerindians)	22	F	+	+	+	+
3	AmeKS	South America (Amerindians)	42	M	+	+	+	+
4	AmeKR	South America (Amerindians)	64	F	+	+	+	+
5	AmeJH	South America (Amerindians)	43	M	+	+	+	+
6	AmeKK	South America (Amerindians)	41	F	+	+	+	+
7	OceMelNC1	Oceania (Melanesians)	64	M	+	−	−	+d
8	OceMelNC2	Oceania (Melanesians)	45	M	+	−	−	+d
9	OceMelNC3	Oceania (Melanesians)	43	M	+	−	−	−
10	OcePolW1c	Oceania (Polynesians)	47	F	+	−	−	+d
11	EurCauC1c	Europe (Caucasians)	26	F	+	+	+	+
12	EurCauC2	Europe (Caucasians)	55	M	+	+	+	+
13	AfrBak541	Africa (Pygmies)	42	M	+	+	+	+
14	AfrBad468	Africa (Bantus)	35	M	+	+	+	+
15	AfrNm51	Africa (Noir-Marrons)	20	F	+	+	+	+
16	AfrNm45	Africa (Noir-Marrons)	20	F	+	+	+	+
17	AfrNm70	Africa (Noir-Marrons)	26	F	+	+	+	+
18	AfrNm020	Africa (Noir-Marrons)	14	F	+	+	+	+
19	Asi-HmLT	Asia (Hmongs)	25	F	+	−	−	−
20	Asi-Hm130	Asia (Hmongs)	28	F	+	−	−	−
21	Asi-Hm225	Asia (Hmongs)	21	F	+	−	−	−
22	Asi-HmYY	Asia (Hmongs)	81	F	+	−	−	−

^aF, female; M, male.

^bThe 1,284-bp fragment corresponds to the 1,468-bp fragment without the noncoding region of fragment C.

^cComplete genome obtained by high-throughput sequencing (see the methodologies and Fig. S1 in the supplemental material).

^dImportantly, in these 3 cases (samples OceMelNC1, OceMelNC2, and OcePolW1), the HTS technique generated the sequences corresponding to fragments B and C. Thus, the complete 1,468-bp fragment for these 3 cases was also obtained.