TABLE 3.
Performance of the UCSC yeast database for Candida and non-Candida clinical isolates prospectively tested by MALDI-TOF MS analysisa
| Organism | No. of isolates tested | No. of isolates with score of: |
||
|---|---|---|---|---|
| >2.0b | ≥1.7 but <2.0c | <1.7d | ||
| Species included in the database | ||||
| C. albicans | 2,924 | 2,882 | 42 | 0 |
| C. glabrata | 642 | 639 | 3 | 0 |
| C. parapsilosis | 210 | 163 | 47 | 0 |
| C. tropicalis | 203 | 176 | 27 | 0 |
| S. cerevisiae | 178 | 157 | 21 | 0 |
| C. krusei | 18 | 8 | 10 | 0 |
| C. norvegensis | 12 | 2 | 10 | 0 |
| C. dubliniensis | 5 | 5 | 0 | 0 |
| C. kefyr | 5 | 2 | 2 | 1 |
| C. neoformans | 5 | 4 | 1 | 0 |
| C. guilliermondii | 3 | 2 | 0 | 1 |
| C. nivariensis | 3 | 2 | 0 | 1 |
| T. inkin | 3 | 1 | 1 | 1 |
| C. lusitaniae | 2 | 0 | 1 | 1 |
| C. lambica | 1 | 0 | 0 | 1 |
| C. pararugosa | 1 | 0 | 0 | 1 |
| C. utilis | 1 | 0 | 0 | 1 |
| G. candidum | 1 | 0 | 0 | 1 |
| L. elongisporus | 1 | 0 | 0 | 1 |
| P. caribbica | 1 | 0 | 0 | 1 |
| P. membranifaciens | 1 | 0 | 0 | 1 |
| T. asteroides | 1 | 0 | 1 | 0 |
| Total | 4,221 | 4,043 | 166 | 12 |
| Species not included in the database | ||||
| Arxiozyma telluris | 1 | 0 | 0 | 1 |
| Candida aaseri | 1 | 0 | 0 | 1 |
| Candida blankii | 1 | 0 | 0 | 1 |
| Candida famata | 1 | 0 | 0 | 1 |
| Candida lipolytica | 1 | 0 | 0 | 1 |
| Candida sloffiae | 1 | 0 | 0 | 1 |
| Malassezia pachydermatis | 1 | 0 | 0 | 1 |
| Pichia fabianii | 1 | 0 | 0 | 1 |
| Rhodotorula glutinis | 1 | 0 | 0 | 1 |
| Rhodotorula sloffiae | 1 | 0 | 0 | 1 |
| Williopsis sp. | 1 | 0 | 0 | 1 |
| Total | 11 | 0 | 0 | 11 |
| Overall species | 4,232 | 4,043 | 166 | 23 |
| ID (%) at the first run | 95.5 | 4.0 | 0.5 | |
| ID (%) at the second run | 99.5 | 0 | 0.5 | |
Isolates were treated with the fast extraction protocol prior to MALDI-TOF MS analysis for identification.
A total of 1,676 (39.6%) isolates with scores of ≥2.3 (high-probable species identification by Bruker Daltonics) were from C. albicans (1,046 isolates), C. glabrata (491 isolates), C. parapsilosis (35 isolates), C. tropicalis (46 isolates), S. cerevisiae (32 isolates), C. krusei (8 isolates), C. norvegensis (2 isolates), C. dubliniensis (5 isolates), C. kefyr (2 isolates), C. neoformans (4 isolates), C. guilliermondii (2 isolates), and C. nivariensis (2 isolates).
Isolates were all identified to the species level after the second run of MALDI-TOF MS analysis.
Isolates were all identified by the rDNA sequence-based method.