TABLE 1.
Gene | Primer sequence (5′ to 3′) | Annealing temperature (°C) | Amplicon size (bp) |
---|---|---|---|
rpoB | CGTGGAGGCGATCACACCGCAGTT | 60 | 215 |
AGTGCGACGGGTGCACGTCGCGGACCT | |||
gyrA | GGTGCTCTATGAAATGTTCG | 60 | 234 |
GCTTCGGTGTACCTCATCG | |||
gyrB | CGATGTTCCAGGCGATACTT | 60 | 163 |
ATCTTGTGGTAGCGCAGCTT | |||
rrs | GTAATCGCAGATCAGCAACG | 60 | 216 |
TTTTCGTGGTGCTCCTTAGAA | |||
tlyA | GTCTCTGGCCGAACTCGAAG | 60 | 1,000 |
ATTGTCGCCCAATACTTTTTCTAC | |||
eis promoter | AAATTCGTCGCTGATTCTCG | 54 | 387 |
CGCGACGAAACTGAGACC | |||
katG | CATGAACGACGTCGAAACAG | 55 | 270 |
CTCTTCGTCAGCTCCCACTC | |||
inhA promoter | AGAAAGGGATCCGTCATGGT | 55 | 340 |
GTCACATTCGACGCCAAAC | |||
ahpC promoter | CACTGCTGAACCACTGCTTT | 55 | 196 |
CAGTGGCATGACTCTCCTCA |