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. 2014 May;52(5):1322–1329. doi: 10.1128/JCM.00857-13

TABLE 1.

Primers used for PCR amplification of nine genes

Gene Primer sequence (5′ to 3′) Annealing temperature (°C) Amplicon size (bp)
rpoB CGTGGAGGCGATCACACCGCAGTT 60 215
AGTGCGACGGGTGCACGTCGCGGACCT
gyrA GGTGCTCTATGAAATGTTCG 60 234
GCTTCGGTGTACCTCATCG
gyrB CGATGTTCCAGGCGATACTT 60 163
ATCTTGTGGTAGCGCAGCTT
rrs GTAATCGCAGATCAGCAACG 60 216
TTTTCGTGGTGCTCCTTAGAA
tlyA GTCTCTGGCCGAACTCGAAG 60 1,000
ATTGTCGCCCAATACTTTTTCTAC
eis promoter AAATTCGTCGCTGATTCTCG 54 387
CGCGACGAAACTGAGACC
katG CATGAACGACGTCGAAACAG 55 270
CTCTTCGTCAGCTCCCACTC
inhA promoter AGAAAGGGATCCGTCATGGT 55 340
GTCACATTCGACGCCAAAC
ahpC promoter CACTGCTGAACCACTGCTTT 55 196
CAGTGGCATGACTCTCCTCA