TABLE 2.

Twelve targeted RRDR mutations in rpoB genes of M. tuberculosis from 18 rifampin-resistant isolates and 59 clinical samples

Position	Mutation	Codon change	Amino acid change	No. of isolates or samples
				RCA		Sequencing		Phenotype (resistance)
				Stored isolate	Clinical sample	Stored isolate	clinical sample	Stored isolate	Recent clinical isolate
514	TTC insertion	TTC- TTCTTC	Phe→PhePhe	1	0	1	0	1	0
516	A→T	GAC→GTC	Asp→Val	0	2	0	2	0	2
516	G→T	GAC→TAC	Asp→Tyr	0	3	0	3	0	3
526	C→A	CAC→AAC	His→Asn	0	5	0	5	0	5
526	C→T	CAC→TAC	His→Tyr	2	4	2	4	2	4
526	C→G	CAC→GAC	His→Asp	0	4	0	4	0	4
526	A→T	CAC→CTC	His→Leu	1	3	1	3	1	3
526	A→C	CAC→CCC	His→Pro	0	5	0	5	0	4
526	A→G	CAC→CGC	His→Arg	1	7	1	7	1	7
531	C→T	TCG→TTG	Ser→Leu	12	24	12	24	12	23
516 and 526	A→T and A→G	GAC→GTC and CAC→CGC	Asp→Val and His→Arg	0	2	0	2	0	2
511 and 516	T→C and A→G	CTG→CCG and GAC→GGC	Leu→Ser and Asp→Gly	1	0	1	0	1	0
NAa	None	None	None	NA	NA	NA	NA	NA	2
Total				18	59	18	59	18	59

^aNA, not applicable.