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Schematic representation of the parental TEV clone (TEV-Ros1), including the visual Ros1 marker (gray rectangle), and the nine recombinant clones in which the NIb gene (black rectangle) was relocated to nine different intercistronic positions of the viral polyprotein (TEVΔNIb-Ros1-NIb1 to -9). Amino acid sequences that are indicated next to the NIb amino or carboxy terminus in some cases (TEVΔNIb-Ros1-NIb1, -2, -3, and -9) were inserted to complement NIb processing from the polyprotein.
