FIG 4.

Anthocyanin accumulation at 15 d.p.i. in systemic leaves of tobacco plants inoculated with TEV recombinant clones in which the NIb gene was relocated to different intercistronic positions. (A) The absorbance at 530 nm of extracts from systemic leaves from three inoculated wild-type and transgenic plants constitutively expressing TEV NIb was plotted. Error bars represent ±1 standard deviation among experimental replicates. Letters a, b, and c over the bars represent groups of inoculations with homogeneous accumulation of anthocyanins according to a Tukey honestly significant difference test. (B) Pictures of systemic leaves harvested at 15 d.p.i. from three wild-type and three transgenic tobacco plants that were noninoculated and inoculated with TEV-Ros1 and TEVΔNIb-Ros1-NIb1 to -3 as indicated.