FIG 2.

SARM siRNA 3-1-GFP or SARM siRNA-negative-control-GFP organization and protein expression. (A) The SARM siRNA-GFP signal in freshly harvested lungs demonstrates polymer formation by the SARM siRNA transgene, i.e., GFP in lungs of transgenic mice, compared to the wild-type control. Images show cross-sections of mouse lung proximal and distal airway sections from transgenic mice or wild-type control mice stained with DAPI as a nuclear stain. Corresponding differential interference contrast/DAPI images are shown. Wild-type images are shown as a control. (B1) Western blotting was used to evaluate the SARM and TRIF expression in wild-type control mice, RSV-infected mice, and RSV-infected mice treated with resveratrol (RES) (lanes 1 to 3). RSV-infected mice treated with RES were either transfected with SARM siRNA negative control (lane 4) or SARM siRNA 3-1 (lane 5). (B2) SARM siRNA reduced the SARM/β-actin ratio in the lungs of RES-treated mice. **, P < 0.01 for RES-treated, SARM siRNA 3-1-transfected mice versus RES-treated, siRNA negative-control-transfected mice. (B3) SARM siRNA increased the TRIF/β-actin ratio in the lungs of RES-treated mice. **, P < 0.01 for RES-treated, SARM siRNA 3-1-transfected mice versus RES-treated, siRNA negative-control-transfected mice. These experiments were performed three times with three mice per group.