FIG 2.

Higher-order interactions formed by crystal packing of H6N6-NS1. (A) The NS1, forming a “dimer” via RBD-RBD interactions, is shown in light yellow for molecule A (Mol A) and bright orange for molecule B (Mol B). Each ED in the NS1 “dimer” is involved in two types of ED-ED interactions (dashed circle) in the crystal (each depicted with respect to the ED of Mol B for clarity) with neighboring NS1 molecules: molecule C (Mol C) in magenta and molecule D (Mol D) in green. (B) The type A ED-ED interaction between the ED of Mol C (magenta) and the ED of Mol B (bright orange) is mediated by the long α-helix of each ED, which links the “dimers” of NS1 into zigzag chains (each NS1 is interchangeably colored magenta and bright orange). (C) The type B ED-ED interactions between the ED of Mol B (bright orange) and the ED of Mol D (green) links NS1 “dimers” into helical chains (each NS1 is interchangeably colored green and bright orange). In panels A and B, the dotted circles highlight the linkages of the NS1 “dimers” into chains created by type A and B ED-ED interactions, respectively.