FIG 3.

Effects of mutations in the ribosomal frameshift signals on HIV-1 titers. Viruses were generated by transfecting producer cell lines with helper plasmids expressing HIV-1 Env and Gag/Gag-Pol and were used to infect HUT/R5 cells. Virus titers were determined using flow cytometry by detecting cells that express HSA and Thy markers. (A) Representative flow cytometry analyses of HUT/R5 cells infected with viruses harvested from producer cells. (B) Relative contribution of Thy⁺ and HSA⁺ cells in all infection events. Total infection events are set as 100%. Error bars represent standard deviations of three infection experiments.