FIG 1.

Characterization of the receptor-binding properties of H6 influenza viruses. (A) Hemagglutination titers of H6 influenza viruses with 0.5% cRBCs treated as follows: cBRCs, untreated; desialylated (Desial) cRBCs, treated with Vibrio cholerae neuraminidase; α-2,6 cRBCs, treated with VCNA and resialylated with α-2,6-glycans. The dashed line indicates the lower limit of detection. (B) Glycan-binding specificity of H6 viruses. The binding of the viruses to two different glycans (α-2,3-glycans, blue; α-2,6-glycans, red) was tested. The data shown are the means of three repeats; the error bars indicate standard deviations. Significant differences were detected between the affinities for the two glycans.