TABLE 3.
Genotype and manifestation in mice of H6 influenza viruses
| Virus | Group of each gene segment in the phylogenetic tree |
Genotype | Virus titers in organs in mice (log10 EID50)a |
Maximum body wt change (%) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HA | NA | PB2 | PB1 | PA | NP | M | NS | Nasal turbinate | Lung | |||
| DK/GD/S1328/10 (H6N2) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | A1 | 0.8 ± 0.4 | 5.8 ± 0.4 | +8.4 |
| GS/GD/S1384/10 (H6N2) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | A1 | 1.8 ± 0.5 | 5.3 ± 0.3 | +10.8 |
| DK/GD/S1289/10 (H6N2) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | A2 | 0.8 ± 0.6 | 5.5 ± 0.3 | +10.0 |
| DK/HuN/S3047/09 (H6N2) | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | A3 | ND | 1.8 ± 0.4 | +11.1 |
| CK/GD/S1453/10 (H6N2) | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 2 | A4 | ND | 5.3 ± 0.3 | +9.6 |
| CK/GD/S1312/10 (H6N2) | 1 | 5 | 1 | 5 | 1 | 1 | 5 | 5 | A5 | 2.3 ± 0.1 | 4.4 ± 0.1 | −6.1 |
| DK/HuB/S4135/10 (H6N2) | 1 | 5 | 6 | 5 | 4 | 5 | 5 | 5 | A6 | 0.7 ± 0.3 | 2.2 ± 0.4 | +8.2 |
| DK/HuB/S4170/08 (H6N2) | 2 | 2 | 5 | 4 | 5 | 2 | 4 | 4 | A7 | ND | 2.9 ± 1 | +13.7 |
| DK/HuB/S1114/09 (H6N2) | 2 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | A8 | 1.4 ± 0.3 | 5.5 ± 0.3 | +8.2 |
| DK/HuN/S1284/09 (H6N2) | 2 | 4 | 1 | 2 | 2 | 1 | 1 | 3 | A9 | 0.7 ± 0.3 | 5.4 ± 0.1 | −1.4 |
| CK/HuN/S4191/09 (H6N2) | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | A10 | 0.7 ± 0.3 | 1.5 | +11.1 |
| DK/HuN/S4386/09 (H6N2) | 3 | 1 | 4 | 1 | 1 | 1 | 1 | 2 | A11 | 0.7 ± 0.3 | 5.4 ± 0.1 | +12.8 |
| DK/HuB/S1366/09 (H6N2) | 3 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | A12 | ND | 5.3 ± 0.4 | +9.7 |
| DK/GD/S4192/08 (H6N2) | 4 | 1 | 1 | 1 | 3 | 1 | 1 | 2 | A13 | 1.3 ± 0.3 | 5.3 ± 0.4 | +7.9 |
| DK/GD/S1566/09 (H6N2) | 4 | 2 | 1 | 1 | 3 | 1 | 1 | 2 | A14 | ND | ND | +10.2 |
| CK/GD/S2346/09 (H6N2) | 5 | 2 | 5 | 4 | 5 | 2 | 4 | 4 | A15 | 1.5 | 5.7 ± 0.1 | +9.2 |
| DK/FJ/S4081/08 (H6N2) | 5 | 2 | 5 | 4 | 5 | 2 | 4 | 4 | A15 | 1.8 ± 0.1 | 5.8 ± 0.6 | +9.3 |
| CK/GD/S1414/10 (H6N6) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | B1 | 1.5 ± 0.3 | 4.8 ± 0.4 | +11.8 |
| DK/ZJ/S1023/10 (H6N6) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | B1 | ND | 5.8 ± 0.6 | +8.9 |
| DK/GD/S3073/10 (H6N6) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | B1 | 2 ± 0.7 | 4.1 ± 0.3 | −3.7 |
| GS/GD/S4362/09 (H6N6) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | B1 | 1.2 ± 0.3 | 5.8 ± 0.5 | +9.3 |
| DK/GD/S1155/11 (H6N6) | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 2 | B2 | 0.8 ± 0.4 | 4.9 ± 0.5 | +6.6 |
| DK/GD/S3180/10 (H6N6) | 1 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | B3 | 0.7 ± 0.3 | 1.6 ± 0.1 | +13.4 |
| DK/HuN/S4273/10 (H6N6) | 1 | 1 | 1 | 3 | 2 | 1 | 1 | 2 | B4 | 2.3 ± 0.1 | 5.5 ± 0.4 | +8.2 |
| CK/GD/S1311/10 (H6N6) | 1 | 1 | 1 | 5 | 1 | 1 | 5 | 5 | B5 | 1.8 ± 0.4 | 5.3 ± 0.1 | −5.7 |
| DK/ZJ/S1134/11 (H6N6) | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | B6 | ND | 5.4 ± 0.4 | +6.1 |
| DK/GD/S3225/10 (H6N6) | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | B6 | 1.2 ± 0.3 | 3.4 ± 0.1 | −5.8 |
| DK/GD/S3468/10 (H6N6) | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | B7 | 0.9 ± 0.4 | 5.6 ± 0.1 | +13.8 |
| CK/HuN/S4495/10 (H6N6) | 1 | 1 | 5 | 3 | 2 | 3 | 1 | 4 | B8 | 2.4 ± 0.9 | 4.4 ± 0.1 | +11.6 |
| CK/HuN/S3003/09 (H6N6) | 1 | 3 | 1 | 1 | 2 | 1 | 3 | 1 | B9 | 0.7 ± 0.3 | 4.0 | +8.2 |
| DK/GD/S4018/10 (H6N6) | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | B10 | 0.6 ± 0.1 | 4.8 ± 0.6 | +10.5 |
| DK/GD/S1663/09 (H6N6) | 2 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | B11 | 1.2 ± 0.6 | 4.3 ± 0.7 | +9.5 |
| CK/GX/S4029/10 (H6N6) | 2 | 3 | 2 | 2 | 1 | 1 | 1 | 1 | B12 | 1.2 ± 0.3 | 2 ± 0.5 | +11.0 |
| CK/GX/S4381/10 (H6N6) | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | B13 | 0.6 ± 0.1 | 1.6 ± 0.1 | +7.4 |
| DK/GX/S4111/10 (H6N6) | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | B13 | 0.7 ± 0.3 | 3.8 ± 0.4 | +9.2 |
| DK/ZJ/S4204/10 (H6N6) | 3 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | B14 | ND | 5.3 ± 0.4 | +9.1 |
| DK/GD/S4251/10 (H6N6) | 5 | 2 | 3 | 2 | 1 | 4 | 2 | 3 | B15 | 3.8 ± 0.4 | 5.3 ± 0.1 | −2.9 |
| DK/GD/S1419/11 (H6N6) | 5 | 2 | 3 | 2 | 1 | 4 | 2 | 3 | B15 | 2.4 ± 0.1 | 5.9 ± 0.5 | −2.5 |
a
Six-week-old BALB/c mice were inoculated intranasally with 106 EID50 of each virus in a 50-μl volume of medium. Three mice from each group were killed on day 3 postinoculation, and virus titers were determined in samples of nasal turbinate, lung, spleen, kidney, and brain in eggs. Virus was not detected from the spleen, kidney, or brain of any mouse; therefore, these data are not shown in the table. Data are presented as means ± standard deviations. ND, virus not detected in sample.