FIG 2.

SFTSV NSs inhibits activation of IFN-β induced by different signaling factors. HEK293T cells were transfected with the indicated viral protein expression plasmids and an IFN-β–CAT plasmid, along with the RIG-I N terminus (RIG-I N) (A), MAVS (B), TBK1 (C), or IRF3-5D (D), and at 16 h after transfection, cell lysates were collected and assessed for CAT activity. Error bars represent the standard deviation of the mean percentage in three independent experiments. Asterisks specify statistically significant differences (P < 0.05) between the indicated group and the pCAGGS-treated group. Expression of the proteins was confirmed by Western blotting using anti-FLAG, anti-HA, anti-IRF3, and anti-β-tubulin antibodies.