FIG 8.

The early endosomal marker Rab5 is associated with the SFTSV NSs-induced cytoplasmic vesicles. HeLa cells were transfected with plasmid carrying the Golgi apparatus marker B4GalT1-RFP (A), the endosomal marker Rab5-RFP (B), or the a dominant negative (DN) variant of Rab5 fused to mCherry (C). At 24 h posttransfection, cells were infected with SFTSV (MOI = 0.5). At 24 h postinfection, the cells were fixed, stained with anti-SFTSV NSs antibody, and analyzed by confocal microscopy. Cell nuclei were visualized by using Hoechst dye. Arrows, sections selected for the insets.