FIG 1.

Characterization of human Hues9 ES cell-derived pNSCs and NPCs. (A) Representative bright-field phase-contrast images of live pNSC and NPC cultures using a 10× objective lens. (B) Expression of N-cadherin (red) in pNSC colonies and lack of expression in NPCs as detected by IFA. (C) Flow cytometry analysis of the expression of Sox2, Pax6, Nestin, and Ki67 in pNSCs. (D) Expression of the neuronal marker βIII-tubulin (green) in pNSCs differentiated for 4 weeks in neuronal differentiation medium, as detected by IFA. (E) Expression of GFAP (green) and S100β (red) in NPCs generated de novo from pNSCs (5 days) or cultured for 5 weeks in matrix-free conditions as neurospheres as detected by IFA. For panels B, D, and E, nuclei were counterstained with Hoechst (blue). IFA images were acquired by epifluorescence microscopy, and those shown are representative of multiple fields. Scale bar, 25 μm.