FIG 7.

IE expression is generally confined to pNSCs with low expression of the FORSE-1 epitope. (A) Immunofluorescence analysis of Hues9 pNSCs and NPCs with the monoclonal antibody FORSE-1 (green). Nuclei were counterstained with Hoechst (blue). Images were acquired by epifluorescence microscopy, and those shown are representative of multiple fields. (B) Immunofluorescence analysis with antibodies to FORSE-1 (green) and viral IE (red) of Hues9 pNSCs infected with TB40E at various MOIs (or mock infected), as indicated on the figure panel, and harvested at 24 h p.i. Images were acquired by epifluorescence microscopy, and those shown are representative of multiple fields. (C) Hues16, Hues6, and H9 pNSCs were seeded and stained with the FORSE-1 antibody as described in panel A. Scale bars, 100 μm.