FIG 1.

E7 proteins augment IL-18BP expression in primary keratinocytes. (A) Primary human foreskin keratinocytes stably expressing vector alone (pBabe-Puro), HPV16 E7, HPV16 E6, or HPV16 E6sE7 were treated with IFN-γ (10 ng/ml) for 16 h. Following treatment, supernatants were harvested and levels of secreted IL-18BP measured by ELISA. (B) Primary human foreskin keratinocytes expressing E7 from HPV6b, HPV11, HPV16, and HPV18 were treated with IFN-γ (10 ng/ml) for 16 h. Following treatment, supernatants were harvested and levels of secreted IL-18BP measured by ELISA. Results are expressed as the means ± standard deviations (SD) from three independent experiments. *, P < 0.05; **, P < 0.01.