FIG 6.

Binding of recombinant MNV-1 P-domains to MAb A6.2. ELISA plates were coated overnight at 4°C with bacterially expressed, recombinant MNV-1 P domain A′B′ loop (A) and E′F′ loop (B) mutants at 50 μg/ml in each well. Diluted purified MAb A6.2 followed by secondary antibodies was incubated for 60 min at 37°C. MNV.CR3 P domain was used as a negative control. Data are presented as means ± SE from three independent experiments. The three bars in each set of bars represent three dilutions of purified MAb A6.2: 1, 0.1, and 0.01 μg/ml.