TABLE 1.

Primers for MNV-1 virus site-directed mutagenesis

Mutation	Direction	Primer sequence (5′→3′)
E296K	Forward	GAGGCTGCCTATGAATTCCAATCGG
	Reverse	CCGATTGGAATTCATAGGCAGCCTC
Q298E	Forward	CTGCCTATGAGTTCGAATCGGGCACC
	Reverse	GGTGCCCGATTCGAACTCATAGGCAG
Q298S	Forward	GCCTATGAGTTCTCATCGGGCACCG
	Reverse	CGGTGCCCGATGAGAACTCATAGGC
S299A	Forward	GCCTATGAGTTCCAAGCGGGCACC
	Reverse	GGTGCCCGCTTGGAACTCATAGGC
S299R	Forward	CTATGAGTTCCAACGGGGCACCGG
	Reverse	CCGGTGCCCCGTTGGAACTCATAG
G300F	Forward	GAGTTCCAATCGTTCACCGGTGAGG
	Reverse	CCTCACCGGTGAACGATTGGAACTC
G300K	Forward	GAGTTCCAATCGAAGACCGGTGAGG
	Reverse	CCTCACCGGTCTTCGATTGGAACTC
G300R	Forward	GAGTTCCAATCGAGGACCGGTGAGG
	Reverse	CCTCACCGGTCCTCGATTGGAACTC
T301I	Nonea
V378A	Forward	GTTCGCCAGCCTCACTGCTGC
	Reverse	GCAGCAGTGAGGCTGGCGAAC
V378F	Forward	CAGGGTGTTCGCCAGCTTCACTGCTGCGGCCTC
	Reverse	GAGGCCGCAGCAGTGAAGCTGGCGAACACCCTG
V378L	Forward	GTGTTCGCCAGCGCCACTGCTGCGG
	Reverse	CCGCAGCAGTGGCGCTGGCGAACAC
A381F	Forward	CAGCGTCACTGCTTTTGCCTCTCTTGAC
	Reverse	GTCAAGAGAGGCAAAAGCAGTGACGCTG
A381G	Forward	GTCACTGCTGGGGCCTCTCTTGACTTG
	Reverse	CAAGTCAAGAGAGGCCCCAGCAGTGAC
A382G	Forward	GTCACTGCTGCGGGCTCTCTTGACTTG
	Reverse	CAAGTCAAGAGAGCCCGCAGCAGTGAC
A382K	Forward	GTCACTGCTGCGAAATCTCTTGACTTGG
	Reverse	CCAAGTCAAGAGATTTCGCAGCAGTGAC
A382P	Forward	GCGTCACTGCTGCGCCCTCTCTTGACTTGG
	Reverse	CCAAGTCAAGAGAGGGCGCAGCAGTGACGC
A382R	Forward	GTCACTGCTGCGCGCTCTCTTGAC
	Reverse	GTCAAGAGAGCGCGCAGCAGTGAC
D385E	Forward	GGCTCTCTTGAGTTGGTGGATGGCAG
	Reverse	CTGCCATCCACCAACTCAAGAGAGCC
L386F	Forward	CTCTCTTGACTTCGTGGATGGCAGGG
	Reverse	CCCTGCCATCCACGAAGTCAAGAGAG

^aMutation arose naturally.