TABLE 3.

Primers for MNV-1 P domain site-directed mutagenesis

Mutation	Direction	Primer sequence (5′→3′)
Y2965K	Forward	GGCTGCCAAAGAGTTCCAATCGGGCACCGGTG
	Reverse	GGAACTCTTTGGCAGCCTCCGCCGCAAAGCAGG
F297A	Forward	GAGGCTGCCTATGAGGCCCAATCGGGCACCGG
	Reverse	CCGGTGCCCGATTGGGCCTCATAGGCAGCCTC
F297I	Forward	CTATGAGATCCAATCGGGCACCGGTGAGGTG
	Reverse	CGATTGGATCTCATAGGCAGCCTCCGCCGC
F297S	Forward	GCGGAGGCTGCCTATGAGTCCCAATCGGGC
	Reverse	GCCCGATTGGGACTCATAGGCAGCCTCCGC
Q298E	Forward	CTGCCTATGAGTTCGAATCGGGCACCGGT
	Reverse	ACCGGTGCCCGATTCGAACTCATAGGCAG
Q298S	Forward	GGCTGCCTATGAGTTCTCATCGGGCACCGGTGAG
	Reverse	CTCACCGGTGCCCGATGAGAACTCATAGGCAGCC
S299A	Forward	GGCTGCCTATGAGTTCCAAGCGGGCACCGG
	Reverse	CCGGTGCCCGCTTGGAACTCATAGGCAGCC
S299R	Forward	GGCTGCCTATGAGTTCCAAAGGGGCACCGGT
	Reverse	ACCGGTGCCCCTTTGGAACTCATAGGCAGCC
G300F	Forward	CTATGAGTTCCAATCGTTCACCGGTGAGGTGGCG
	Reverse	CGCCACCTCACCGGTGAACGATTGGAACTCATAG
G300K	Forward	CCTATGAGTTCCAATCGAAGACCGGTGAGGTGGCGAC
	Reverse	GTCGCCACCTCACCGGTCTTCGATTGGAACTCATAGG
G300R	Forward	ATGAGTTCCAATCGCGCACCGGTGAGGTG
	Reverse	CACCTCACCGGTGCGCGATTGGAACTCAT
V378A	Forward	TGTTCGCCAGCGCCACTGCTGCGGC
	Reverse	GCCGCAGCAGTGGCGCTGGCGAACA
V378L	Forward	GTGTTCGCCAGCCTCACTGCTGCGG
	Reverse	CCGCAGCAGTGAGGCTGGCGAACAC
A381F	Forward	GTTCGCCAGCGTCACTGCTTTCGCCTCTCTTGACTTGG
	Reverse	CCAAGTCAAGAGAGGCGAAAGCAGTGACGCTGGCGAAC
A381G	Forward	AGCGTCACTGCTGGGGCCTCTCTTGAC
	Reverse	GTCAAGAGAGGCCCCAGCAGTGACGCT
A382G	Forward	GCTGCGGGGTCTCTTGACTTGGTGGATGGCAGG
	Reverse	CAAGAGACCCCGCAGCAGTGACGCTGGCGAACACC
A382K	Forward	CCAGCGTCACTGCTGCGAAGTCTCTTGACTTGGTGGA
	Reverse	TCCACCAAGTCAAGAGACTTCGCAGCAGTGACGCTGG
A382P	Forward	GCGTCACTGCTGCGCCCTCTCTTGACTTG
	Reverse	CAAGTCAAGAGAGGGCGCAGCAGTGACGC
A382R	Forward	GCGTCACTGCTGCGCGCTCTCTTGACTTGG
	Reverse	CCAAGTCAAGAGAGCGCGCAGCAGTGACGC
D385E	Forward	GCGGCCTCTCTTGAATTGGTGGATGGCAG
	Reverse	CTGCCATCCACCAATTCAAGAGAGGCCGC