TABLE 4.

List of mutations in MNV-1 P domains

Amino acid mutation(s)	Descriptiona
A′B′ loop
E296Kb	Same size, added charge
Q298E	Same size, reduced charge
Q298S	Smaller, still polar
S299A	Smaller, no clash
S299R	Larger, added charge
G300F	Larger, hydrophobic
G300K	Larger, added charge
G300R	Larger, added charge
T301I	Same size, same charge
E′F′ loop
V378A	Smaller, less hydrophobic
V378Fc	Larger, more hydrophobic
V378L	Same size, same charge
A381F	Larger, more hydrophobic
A381G	Smaller, flexible
A382G	Smaller, no clash
A382K	Larger, added charge
A382P	Larger, structural disruptor
A382R	Larger, added charge
D385E	Same size, same charge
D385Gd	Smaller, added charge
L386Fe	Larger, more hydrophobic
L378F D385Gc	Double mutant

^aSize and charge comparison between the mutants and wild-type MNV-1.

^bPublished virulence determinant in MNV-1.CW1 (40).

^cDominant mutation in passage 5.

^dDominant mutation in passages 3 and 4.

^ePublished MAb A6.2 escape MNV-1 mutation (30).