TABLE 5.
Summary of MAb A6.2 binding results to the MNV-1 P domain
| Virus | Result by: |
||
|---|---|---|---|
| ELISAa | Western blot (%) | Biacore | |
| WT | + | 100 | 4.6 × 10−8 |
| CR3 (negative control) | − | 0 | 0 |
| Mutants | |||
| Y295K | − | 0 | 0 |
| F297A | + | 0 | 0 |
| F297S | − | 9 | 0 |
| F297I | +/− | 52 | 0 |
| Q298E | + | 67 | 0 |
| Q298S | − | 12 | 1.5 × 10−8 |
| S299A | + | 114 | 5.0 × 10−8 |
| S299R | + | 139 | 2.5 × 10−8 |
| G300F | − | 29 | 0 |
| G300K | + | 116 | 8.0 × 10−7 |
| G300R | +/− | 19 | 0 |
| V378A | +/− | 48 | 1.3 × 10−6 |
| V378L | + | 216 | 1.3 × 10−6 |
| A381F | +/− | 32 | 0 |
| A381G | + | 155 | 7.1 × 10−8 |
| A382G | − | 0 | 0 |
| A382K | − | 0 | 0 |
| A382P | + | 60 | 0 |
| A382R | − | 0 | 0 |
| D385E | − | 0 | 0 |
a
+, signal within the error of wild-type protein; −, signal within error of controls; +/−, intermediate result.