FIG 4.

BGLF2 induces p21 protein levels in a p53-independent manner. (A) Fucci cells were transfected with pMZS3F or pMZS3F-BGLF2 and, 24 h later, cell lysates were analyzed by Western blotting for p53, phosphorylated p53 [p-p53 (ser15)], and p21, using actin as a loading control. (B and C) H1299 p53-null cells were transfected as described for panel A and then lysates were Western blotted for p21 (B) or EAPP (C) in addition to FLAG-BGLF2 and actin. CNE2Z (D) and H1299 (E) cells were transfected as described above, and then p21 transcript levels were determined by quantitative PCR and normalized to GAPDH. Average values with standard deviations are shown from three independent experiments.