FIG 6.
Localization of MMTV subviral RNA in infected cells. (A) Schematic diagram depicting the MMTV subviral RNA (svRNA) construct, which is driven by the CMV promoter and contains R, U5, the first 746 nucleotides of gag (Ψ), the Rem response element (RemRE), and four BoxB stem-loops, which are bound by the λN-eGFP reporter. The λN-eGFP reporter contains an M9 nuclear localization signal (NLS) (74). (B) Expression of the λN-eGFP reporter alone in MMTV(C3H)-infected NMuMG (NMuMG.C3H) cells. (C) Two planes through the same NMuMG.C3H cell transfected with svRNA and λN-eGFP and immunostained for Gag. The svRNA and Gag colocalize in cytoplasmic complexes. White boxes indicate the areas shown in enlargements. (D) MMTV-infected NMuMG cells were transfected with λN-eGFP and immunostained for Gag and YB-1, G3BP1, or TIA-1.