FIG 2.

Transcription of ERVWE1 and GCM1 in response to virus infection. (A) Relative levels of ERVWE1 transcripts (env exon transcripts) and transcripts encoding GCM1 were measured in uninfected and influenza A/WSN/33 virus-infected (MOI = 0.5) CCF-STTG1 cells at 24 h postinfection, primary human fibroblasts at 48 h postinfection, and JEG-3 cells at 24 h postinfection. (B) Levels of ERVWE1 and GCM1 transcripts in CCF-STTG1 cells treated with siRNA targeted against transcripts encoding GCM1 and subsequently infected with influenza A/WSN/33 virus for 24 h relative to those in cells treated with control siRNA and infected with influenza A/WSN/33 virus. (C) Relative levels of transcripts encoding segment 8 of the influenza A/WSN/33 virus in CCF-STTG1 cells treated with scrambled control siRNA or no siRNA reagents and in cells treated with siRNA targeted against transcripts encoding GCM1. siRNA analysis data are presented as means ± SEMs from four independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.