FIG 3.

One-step growth curves and RNA synthesis of each mutant and the WT. BHK-21 and IBRS-2 cells were infected with mutant or WT virus at an MOI of 10. The resulting virus was harvested at different times, titers were determined and expressed as a TCID₅₀ dose (A to D), and the genome copy numbers were measured (E to H) for the same samples by real-time RT-PCR. The mean values ± standard deviations are shown (n = 3) (no significant differences were identified by repeated-measures ANOVA).